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In vivo Protein Biosensors

Description 
"In Vivo Biosensing" is an emerging field in which disease-related biomarkers (including ions, metabolites and proteins) are monitored in a continuous and real-time manner inside the body. These biosensors could address important challenges ranging from improving the fundamental understanding of dynamic physiological processes in vivo, through to continuous monitoring of critical disease-related biomarkers (e.g. troponins – heart attacks, procalcitonin – sepsis, therapeutic drug monitoring, etc). We have recently engineered dye-labelled antibody fragments against EGFR and troponin-I which, upon binding to the target protein, change their fluorescence spectra in a quantifiable manner. The next step in the research program is to immobilise the engineered antibodies onto nanoparticles and inject these “tattoos” into the skin (or other organs), monitoring the tattoo using hand-held fluorescent readers. Using nanoparticles sourced either from our lab or from commercial sources, the student will design and express the antibody fragments, create the tattoos, and test them in animal models of disease. A strong background in protein expression and protein biochemistry is essential, and additional experience in animal studies would be beneficial (but not necessary).
Essential criteria: 
Minimum entry requirements can be found here: https://www.monash.edu/admissions/entry-requirements/minimum
Keywords 
Proteins, protein engineering, biomedical engineering, biosensors, biochemistry, antibodies, disease markers,
School 
Biomedicine Discovery Institute (School of Biomedical Sciences) » Microbiology
Available options 
PhD/Doctorate
Masters by research
Joint PhD/Exchange Program
Time commitment 
Full-time
Top-up scholarship funding available 
No
Physical location 
Monash Clayton Campus
Co-supervisors 
Dr 
Simon Corrie, ARC Centre for Bio-Nano Science

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