You are here

Understanding the biosynthesis of peptide antibiotics by non-ribosomal peptide synthesis

Description 
Non-ribosomal peptide synthesis is a complex biosynthetic process that produces many medically important peptide natural products, including several classes of vital antibiotics such as the glycopeptide antibiotics. As many of the structures of these antibiotics are too complex to produce commercially via chemical synthesis, our ability to access these and related compounds for use in the clinic depends on natural biosynthesis. Thus, gaining an understanding of the enzymatic machineries that make these complex antibiotics in vivo is vital for our ability to generate new compounds to continue the fight against resistant bacterial infections. This project seeks to understand the non-ribosomal peptide synthesise machinery that is central to the synthesis of these antibiotics through a series of biochemical and structural investigations of this modular peptide assembly line. The project will combine protein expression and purification, enzymology, analysing protein-protein interactions and biochemistry together with structural biology techniques such as X-ray crystallography and cryo electron microscopy (Cryo-EM).
Essential criteria: 
Minimum entry requirements can be found here: https://www.monash.edu/admissions/entry-requirements/minimum
Keywords 
antibiotics, peptides, protein expression and purification, enzymology, protein-protein interactions, structural biology, biochemistry cryo-EM, crystallography
Available options 
PhD/Doctorate
Masters by research
Honours
Time commitment 
Full-time
Top-up scholarship funding available 
No
Physical location 
15 Innovation Walk
Co-supervisors 
Dr 
Thierry Izore

Want to apply for this project? Submit an Expression of Interest by clicking on Contact the researcher.