The global burden of peripheral artery disease is at a dramatic increase to due the prevalence of aging, obesity, diabetes, cardiovascular disorders, and autoimmune diseases. Stem cells have a significant promise for cell therapies and regenerative medicine applications. Stem cells serve as bio-factories releasing bioactive products including growth factors and exosomes and there is now increasing evidence that exosomes confer the therapeutic benefits of stem cells, thus accelerating the pathway for cell-free therapies. This project propose aims to enhance vascular regeneration potential of stem cell-derived exosomes by using cues from the cellular environment. Stem cells are highly sensitive to physical stimuli from their surrounding microenvironment and this project will evaluate this by comparing the traditional 2D static culture with dynamic 3D culture. Techniques employed include stem cell culture, immunofluorescence, proliferation assay, 3D culture, exosomes isolation, Western blotting, nanoparticle tracking analysis, and angiogenesis assays.
stem cells, exosomes, regenerative medicine, angiogenesis
School of Clinical Sciences at Monash Health / Hudson Institute of Medical Research
Top-up scholarship funding available
Monash Health Translation Precinct (Monash Medical Centre)